Journal: Viruses

Article Title: Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay

doi: 10.3390/v13091771

Figure Lengend Snippet: Comparison of non-structural protein 1 sequences among closely related flaviviruses. ( A ): Ribbon model highlighting regions of the NS1 protein containing segments exposed at the outer surface to the host environment. ( B ): Sequence comparison showing regions with high sequence disparity. Amino acids depicted in red differ from the corresponding ZIKV NS1 amino acids. A represents positions with two sequences with amino acids identical to ZIKV NS1. The boxes highlight highly conserved sequences, amino acids 117–119 and 227–229, that were mutated to alanine in immunodominant regions 2 and 3.

Article Snippet: Antiserum against ZIKV rNS1 proteins was generated via immunization of goats with wild-type NS1 and NS1 with alanine substitution mutations of amino acids 117–119 or 227–229 were each inoculated in goats. (ProSci, San Diego, CA, USA).

Techniques: Comparison, Sequencing

Journal: Viruses

Article Title: Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay

doi: 10.3390/v13091771

Figure Lengend Snippet: Western blot of NS1 mutants. ( A ): The blot was probed with anti-His6 antibody targeted toward the protein N-terminus. ( B ): The blot was probed with anti-ZIKV NS1 monoclonal antibody targeted toward the C-terminus. Uncropped gels are displayed in .

Article Snippet: Antiserum against ZIKV rNS1 proteins was generated via immunization of goats with wild-type NS1 and NS1 with alanine substitution mutations of amino acids 117–119 or 227–229 were each inoculated in goats. (ProSci, San Diego, CA, USA).

Techniques: Western Blot

Journal: Viruses

Article Title: Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay

doi: 10.3390/v13091771

Figure Lengend Snippet: Ratios of mutant vs. wild-type binding of NS1 by patient samples. The blue line at 1 indicates equal binding. Numbers over 1 indicate increased binding to the named mutant, while under 1 indicate increased wild-type binding. ( A ): Ratios of mutant/wild-type binding from IgG in samples show preferential binding to 117–119 mutant NS1. ( B ): IgM shows preferential binding of mutant NS1 proteins in samples from the Dominican Republic. Colombia = Colombia samples from suspected ZIKV infection. Dom Rep = Dominican Republic samples from suspected ZIKV infection. 117–119 = ZIKV NS1 W117A, G118A, K119A. 227–229 = ZIKV NS1 H227A, T228A, L229A Comparisons were made using Kruskal–Wallis ANOVA. Asterisks represent significant comparisons (**** p < 0.0001; ** p < 0.01). The full dataset is presented in .

Article Snippet: Antiserum against ZIKV rNS1 proteins was generated via immunization of goats with wild-type NS1 and NS1 with alanine substitution mutations of amino acids 117–119 or 227–229 were each inoculated in goats. (ProSci, San Diego, CA, USA).

Techniques: Mutagenesis, Binding Assay, Infection

Journal: Viruses

Article Title: Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay

doi: 10.3390/v13091771

Figure Lengend Snippet: Serum from goats immunized with ZIKV mutants show differential binding to NS1 proteins. Antibody-capture ELISA was performed on a mixture of serum from two goats immunized with ZIKV NS1 protein. Binding of WT ZIKV NS1 showed lower binding to WT ( A ), both mutants ( B , C ), as well as DENV2 WT NS1 ( D ). Data represent samples run in duplicate. Error bars (standard error of the mean) were smaller than the symbols as drawn. The experiment was repeated a total of three times. The legend in panel ( D ) also applies to panels ( A – C ).

Article Snippet: Antiserum against ZIKV rNS1 proteins was generated via immunization of goats with wild-type NS1 and NS1 with alanine substitution mutations of amino acids 117–119 or 227–229 were each inoculated in goats. (ProSci, San Diego, CA, USA).

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Protein Binding

Journal: Viruses

Article Title: Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay

doi: 10.3390/v13091771

Figure Lengend Snippet: Process for purifying and cross-adsorbing ZIKV NS1 antibodies. ZIKV NS1 antibodies were affinity purified using a gravity flow column. ( A ): The bound and eluted fraction was put through a column with DENV NS1 twice. The unbound and eluted (non-DENV reactive) fraction is the cross-adsorbed, ZIKV-specific fraction. ( B ): Antibodies flowing through the column initially bind ZIKV at low signal strength. Following ZIKV NS1 column elution, strength of binding of the solution goes up, but DENV NS1 reactivity is present. Upon cross-adsorption against DENV NS1, specificity of the pAb solution goes up, as well as its binding avidity to ZIKV NS1.

Article Snippet: Antiserum against ZIKV rNS1 proteins was generated via immunization of goats with wild-type NS1 and NS1 with alanine substitution mutations of amino acids 117–119 or 227–229 were each inoculated in goats. (ProSci, San Diego, CA, USA).

Techniques: Affinity Purification, Binding Assay, Adsorption

Journal: Viruses

Article Title: Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay

doi: 10.3390/v13091771

Figure Lengend Snippet: ZIKV NS1 antigen-capture ELISA limit of detection and dynamic range. ( A ): ZIKV rNS1 shows binding significantly higher than BSA down to 7.8 ng/mL. The limit of detection of the assay is between 7.8 and 1.95 ng/mL NS1. ( B ): Linear regression of NS1 detection via ELISA demonstrates assay can quantify protein across a range of values. Comparisons were made using two-way ANOVA with a Holm–Sidak multiple comparisons test. Asterisks represent significant comparisons (**** p < 0.0001; * p < 0.05). Error bars represent the standard error of the mean.

Article Snippet: Antiserum against ZIKV rNS1 proteins was generated via immunization of goats with wild-type NS1 and NS1 with alanine substitution mutations of amino acids 117–119 or 227–229 were each inoculated in goats. (ProSci, San Diego, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: Viruses

Article Title: Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay

doi: 10.3390/v13091771

Figure Lengend Snippet: Production of flavivirus NS1. Coomassie-stained SDS-PAGE images of induced (I) and uninduced (U) cultures from NS1 proteins of yellow fever virus ( A ), West Nile virus ( A , B ) and St. Louis Encephalitis virus ( B ). Bands indicate proteins similar to DENV and ZIKV NS1 WT production. Results from three different colonies [ , , ] for each flavivirus NS1 are indicated. Uncropped gels are displayed in .

Article Snippet: Antiserum against ZIKV rNS1 proteins was generated via immunization of goats with wild-type NS1 and NS1 with alanine substitution mutations of amino acids 117–119 or 227–229 were each inoculated in goats. (ProSci, San Diego, CA, USA).

Techniques: Staining, SDS Page, Virus

Journal: Viruses

Article Title: Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay

doi: 10.3390/v13091771

Figure Lengend Snippet: The ZIKV NS1 antigen-capture assay demonstrates low cross-reactivity to related viruses. Recombinant NS1 antigens were produced and assayed. A: Even at low levels of NS1 detection, the assay is specific to ZIKV NS1. Comparisons made using two-way ANOVA with a Holm–Sidak multiple comparisons test. Asterisks represent significant comparisons between ZIKV NS1 and all other NS1 proteins (**** p < 0.0001; * p < 0.05). Error bars represent the standard error of the mean.

Article Snippet: Antiserum against ZIKV rNS1 proteins was generated via immunization of goats with wild-type NS1 and NS1 with alanine substitution mutations of amino acids 117–119 or 227–229 were each inoculated in goats. (ProSci, San Diego, CA, USA).

Techniques: Recombinant, Produced

Journal: Viruses

Article Title: Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay

doi: 10.3390/v13091771

Figure Lengend Snippet: ZIKV NS1 antigen-capture ELISA results from patients infected during the 2015–2016 outbreaks in Colombia and the Dominican Republic 1 .

Article Snippet: Antiserum against ZIKV rNS1 proteins was generated via immunization of goats with wild-type NS1 and NS1 with alanine substitution mutations of amino acids 117–119 or 227–229 were each inoculated in goats. (ProSci, San Diego, CA, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Infection